| List of services |
| Biomolecular characterization and interaction |
Calorimetric titration + automated software data evaluation.
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method)
Data evaluation - by automated software data evaluation - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site
Booking of the service is available via
CEITEC intranet system
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Calorimetric titration + expert data evaluation + data evaluation training AutoITC200
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method)
Data evaluation - manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model.
Determination of kinetic parameters Km and kcat.
Data evaluation training.
Booking of the service is available via
CEITEC intranet system
|
Calorimetric titration + expert data evaluation
Calorimetric measurement of receptor-ligand interaction (Standard titration method, Single injection method)
Data evaluation - manually by expert - complete thermodynamic profile determination (association constant, enthalpy, entropy, stoichiometry value) using curve fitting models: One set of binding site, Two sets of binding sites, Sequential Binding model.
Determination of kinetic parameters Km and kcat.
Booking of the service is available via
CEITEC intranet system
|
Microdeposition system for biosensor arrays - advanced optimization service
Compact automated non-contact dispensing system of ultra-low volumes of biomolecules and bioreagens. Advanced service involves advanced optimization of instrument settings in case of more complex and viscous samples and reagens.
Booking of the service is available via
CEITEC intranet system
|
Sedimentation velocity
Sedimentation velocity method can be used for determination of sample
dispersity, oligomeric state of proteins, the shape of the particle and it is
also suitable for analysis of aggregates and a study of biomolecular
interactions.
Booking of the service is available via
CEITEC intranet system
|
Sedimentation equilibrium
Sedimentation equilibrium method can be used for accurate determination of
molecular weights of various biomolecules and the study of their interactions.
Booking of the service is available via
CEITEC intranet system
|
Microdeposition system for biosensor arrays - standard service
Compact automated non-contact dispensing system of ultra-low volumes of biomolecules and bioreagens.
Booking of the service is available via
CEITEC intranet system
|
| Crystallization of biomolecules |
Standard crystallization screen set-up
Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids) - Sitting drop crystallization method, Crystallization under oil
Booking of the service is available via
CEITEC intranet system
|
Customized crystallization
Setting-up multidimensional gradients for optimization (from up to 5 different solutions).
Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil.
Booking of the service is available via
CEITEC intranet system
|
Advanced crystallization techniques
Optimization of protein crystal production in large scale (manual setting) - Membrane protein crystallization techniques, Crystallization by dialysis or using capilaries
Note: Service price is calculated individually. Contact the responsible person for details.
Booking of the service is available via
CEITEC intranet system
|
Customized crystallization, Plate storage and inspection
Setting-up multidimensional gradients for optimization (from up to 5 different solutions).
Set-up of optimization screens - Hanging drop crystallization method, Sitting drop crystallization method, Crystallization under oil.
Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C.
Automatic screening of crystals - Regular automatic inspection within the period of one month, UV imaging to distinguish a protein from a salt.
Online access to the images 24/7
Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Booking of the service is available via
CEITEC intranet system
|
Standard crystallization Screen Set-up, Plate Storage and Inspection
Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil.
} Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C
Automatic screening of crystals .
Regular automatic inspection within the period of one month.
UV imaging to distinguish a protein from a salt.
Online access to the images.
Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection
Booking of the service is available via
CEITEC intranet system
|
Plate storage and inspection
Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C.
Automatic screening of crystals. Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images 24/7.
Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection.
Booking of the service is available via
CEITEC intranet system
|
| Others |
Consulting - assistance
Service includes consulting with specialist, expert data evaluation, advanced experimental design or personal user support during measurement.
Booking of the service is available via
CEITEC intranet system
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Initial training for instruments in user mode (operated by users him-herself)
Initial training for instruments which are operated by users him/herself. This includes Biacore T200 (SPR), multichannel SPR imaging system, DelsaMax Core (DLS), Jasco J-815 (CD), VP-ITC, VP-DSC, Monolith NT.115 Nano (MST), Prometheus (DSF), microdeposition system for biosensor arrays and gradient incubator for optimizing protein crystallization temperature.
Training is performed by CF: BIC staff - responsible person for each instrument.
Without this training users cannot operated the instruments on their own.
Booking of the service is available via
CEITEC intranet system
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