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Home page/Core Facilities/Biomolecular Interactions and Crystallization/Automated calorimetric system Auto-iTC200

Automated calorimetric system Auto-iTC200

Name: Automated calorimetric system Auto-iTC200
Technology / Methodology: Biomolecular characterization and interaction
Location: CEITEC Masaryk University
Instrument description: Fully automated isothermal calorimeter AutoITC200 with ultrasmall working cell (200 ul). ITC method is used for characterization of biomolecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and others. Enzyme kinetics, biological activity or the effect of molecular structure changes on binding mechanism can be also assessed. Complete thermodynamic profile of the molecular interaction in a single experiment (stoichiometry, Ka, ∆H and ∆S values) or kinetics parameters Km and kcat can be determined. Auto-iTC200 is designed to measure the heat of binding. In a typical arrangement, the titrant, also referred as the ligand, is injected into the sample cell containing the macromolecule sample material. The calorimetric measurement can be done over a range of biologically relevant conditions (temperature, salt, pH, etc.). ITC system directly measure submilimolar to nanomolar binding constants (10^3 - 10^9 M-1). Measure of the interactions with nanomolar to picomolar binding constants (10^9-10^12 M-1) can be measured using the competitive binding technique, the same principle can be used for low affinity interactions (10^3-10^2 M-1). The operating temperature range is of 2°C to 80°C. Measurement at AutoITC200 is performed exclusively by core facility staff.
Research group: CF: Core Facility Biomolecular Interaction and Crystalization
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Automated calorimetric system Auto-iTC200

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