Quantifoil Cu 300-mesh R2/1, Quantifoil Cu 200-mesh R2/1, Quantifoil Au 200-mesh R2/1, C-flat Cu 200-mesh R2/1, C-flat Cu 400-mesh R2/1, Krios autogrid
Location:
CEITEC Masaryk University
Instrument description:
Transmission electron microscope (TEM) operating at 80-300 kV that is equipped with a direct detector (Falcon II). This microscope allows automated data acquisition of purified proteins and their complexes to the highest possible resolutions (~3A} nowadays. Additionally, the microscope is also equipped with a Gatan energy filter with a 4k CCD camera, which allows elimination of inelastically scattered electrons. The microscope is therefore suitable for cryo-electron tomography of pleiomorfic protein complexes and cellular compartments. Data collection is fully automated by programs EPU (single particle analysis) and Tomo (tilt series).
Research group:
CF: Core Facility Cryo-Electron Microscopy and Tomography
