Core Facility Nanobiotechnology

Main Activity

Scanning probe microscopies for imaging of biological samples - tissue slices, cells, single biomolecules as nucleic acids and proteins. Studies of affinity interactions at the molecular level.

Unique Features

• Visualization and studies of samples using atomic force microscopy (AFM) both in dry state and in liquid

• AFM combined with other techniques: electrochemistry, optical and confocal fluorescence microscopy

• Nanolithography, nanomechanical manipulations, ink-jet based deposition

• Preparation of samples for AFM

• AFM measurements, statistical analysis and data filtration

• Production and bioconjugation of nanoparticles

Key Equipment

• scanning probe microscope - Ntegra Vita / Solaris (NTMDT)

• atomic force microscope NanoWizzard3 (JPK)

• ForceRobot 300 (JPK)

• automated system SolverNEXT (NTMDT)

• ink-jet based deposition system S3 (Scienion)

The Core Facility is part of Czech National Affiliated Centre of INSTRUCT. 

All CEITEC core facilities are available to external users (academia and companies). Czech and international researchers from universities and research institutes interested in accessing core facilities can benefit from support of research infrastructure CIISB, funded by the Ministry of Education, Youth and Sports of the Czech Republic.

  

 

list / cards

Name and position	E-mail	Phone
Petr Skládal Research Group Leader		+420 54949 7659, +420 54949 7010
Jan Přibyl, Ph.D. Researcher		+420 54949 5606
Karel Lacina, Ph.D. Researcher		+420 54949 7611
Veronika Horáčková PhD student		+420 54949 7676
Matěj Pastucha PhD student		+420 54949 8517
Zdeněk Farka PhD student		+420 54949 7674
Guido Caluori odborný pracovník ve výzkumu - postdoc

SELECTED PUBLICATIONS

2017

• FARKA, Z; JURIIK, T; KOVAAR, D; TRNKOVA, L; SKLADAL, P, 2017:Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges. CHEMICAL REVIEWS
• FARKA, Z; MICKERT, MJ; HLAVACEK, A; SKLADAL, P; GORRIS, HH, 2017:Single Molecule Upconversion-Linked Immunosorbent Assay with Extended Dynamic Range for the Sensitive Detection of Diagnostic Biomarkers. ANALYTICAL CHEMISTRY 89 (21), p. 11825 - 11830.
• HLAVACEK, A; PETEREK, M; FARKA, Z; MICKERT, MJ; PRECHTL, L; KNOPP, D; GORRIS, HH, 2017:Rapid single-step upconversion-linked immunosorbent assay for diclofenac. MICROCHIMICA ACTA 184 (10), p. 4159 - 4165.
• LACINA, K; KUBESA, O; VANYSEK, P; HORACKOVA, V; MORAVEC, Z; SKLADAL, P, 2017:Selective electrocatalysis of reduced graphene oxide towards hydrogen peroxide aiming oxidases-based biosensing: Caution while interpreting. ELECTROCHIMICA ACTA 223 , p. 1 - 7.
• NARDONE, G; LA CRUZ, JOD; VRBSKY, J; MARTINI, C; PRIBYL, J; SKLADAL, P; PESL, M; CALUORI, G; PAGLIARI, S; MARTINO, F; MACECKOVA, Z; HAJDUCH, M; SANZ-GARCIA, A; PUGNO, NM; STOKIN, GB; FORTE, G, 2017:YAP regulates cell mechanics by controlling focal adhesion assembly. NATURE COMMUNICATIONS 8
• SKARKOVA, P; NOVOTNY, K; LUBAL, P; JEBAVA, A; PORIZKA, P; KLUS, J; FARKA, Z; HRDLICKA, A; KAISER, J, 2017:2d distribution mapping of quantum dots injected onto filtration paper by laser-induced breakdown spectroscopy. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY 131 , p. 107 - 114.

2016

• CUNDERLOVA, V; HLAVACEK, A; HORNAKOVA, V; PETEREK, M; NEMECEK, D; HAMPL, A; EYER, L; SKLADAL, P, 2016:Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays. MICROCHIMICA ACTA 183 (2), p. 651 - 658.
• FARKA, Z; JURIK, T; PASTUCHA, M; KOVAR, D; LACINA, K; SKLADAL, P, 2016:Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. ELECTROANALYSIS 28 (8), p. 1803 - 1809.
• FARKA, Z; JURIK, T; PASTUCHA, M; SKLADAL, P, 2016:Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk. ANALYTICAL CHEMISTRY 88 (23), p. 11830 - 11836.
• HLAVACEK, A; FARKA, Z; HUBNER, M; HORNAKOVA, V; NEMECEK, D; NIESSNER, R; SKLADAL, P; KNOPP, D; GORRIS, HH, 2016:Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. ANALYTICAL CHEMISTRY 88 (11), p. 6011 - 6017.
• JURIK, T; PODESVA, P; FARKA, Z; KOVAR, D; SKLADAL, P; FORET, F, 2016:Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum. ELECTROCHIMICA ACTA 188 , p. 277 - 285.
• LACINA, K; ZAK, J; SOPOUSEK, J; SZABO, Z; VACLAVEK, T; ZERAVIK, J; FIALA, P; SKLADAL, P, 2016:Transistor Amplifier as an Electrochemical Transducer with Intuitive Optical Read-out: Improving Its Performance with Simple Electronic Solutions. ELECTROCHIMICA ACTA 216 , p. 147 - 151.
• MAKHNEVA, E; MANAKHOV, A; SKLADAL, P; ZAJICKOVA, L, 2016:Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions. SURFACE & COATINGS TECHNOLOGY 290 , p. 116 - 123.
• MANAKHOV, A; MAKHNEVA, E; SKLADAL, P; NECAS, D; CECHAL, J; KALINA, L; ELIAS, M; ZAJICKOVA, L, 2016:The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. APPLIED SURFACE SCIENCE 360 , p. 28 - 36.
• PESL, M; PRIBYL, J; ACIMOVIC, I; VILOTIC, A; JELINKOVA, S; SALYKIN, A; LACAMPAGNE, A; DVORAK, P; MELI, AC; SKLADAL, P; ROTREKL, V, 2016:Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing. BIOSENSORS & BIOELECTRONICS 85 , p. 751 - 757.
• SEDLMEIER, A; HLAVACEK, A; BIRNER, L; MICKERT, MJ; MUHR, V; HIRSCH, T; CORSTJENS, PLAM; TANKE, HJ; SOUKKA, T; GORRIS, HH, 2016:Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. ANALYTICAL CHEMISTRY 88 (3), p. 1835 - 1841.
• ZERAVIK, J; FOHLEROVA, Z; MILOVANOVIC, M; KUBESA, O; ZEISBERGEROVA, M; LACINA, K; PETROVIC, A; GLATZ, Z; SKLADAL, P, 2016:Various instrumental approaches for determination of organic acids in wines. FOOD CHEMISTRY 194 , p. 432 - 440.

2015

• FARKA, Z; KOVAR, D; SKLADAL, P, 2015:Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. SENSORS 15 (1), p. 79 - 92.
• LACINA, K; SKLADAL, P, 2015:Bipolar transistor amplifier for transduction of electrochemical response to visual perception. SENSORS AND ACTUATORS B-CHEMICAL 210 , p. 183 - 189.
• SUN, XL; LACINA, K; RAMSAMY, EC; FLOWER, SE; FOSSEY, JS; QIAN, XH; ANSLYN, EV; BULL, SD; JAMES, TD, 2015:Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite. CHEMICAL SCIENCE 6 (5), p. 2963 - 2967.

2014

• HLAVACEK, A; SEDLMEIER, A; SKLADAL, P; GORRIS, HH, 2014:Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates. ACS APPLIED MATERIALS & INTERFACES 6 (9), p. 6930 - 6935.
• KOVAR, D; FARKA, Z; SKLADAL, P, 2014:Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor. ANALYTICAL CHEMISTRY 86 (17), p. 8680 - 8686.
• MANAKHOV, A; SKLADAL, P; NECAS, D; CECHAL, J; POLCAK, J; ELIAS, M; ZAJICKOVA, L, 2014:Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application. PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE 211 (12), p. 2801 - 2808.
• PESL, M; IVANA, A; PRIBYL, J; HEZOVA, R; VILOTIC, A; AIMOND, F; FAUCONNIER, J; VRBSKY, J; KRUZLIAK, P; SKLADAL, P; KARA, T; ROTREKL, V; LACAMPAGNE, A; DVORAK, P; MELI, AC, 2014:Molecular and Functional Characterization of Uniform-Sized Beating Embryoid Bodies and Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells. BIOPHYSICAL JOURNAL 106 (2), p. 565A - 565A.

2013

• FARKA, Z; KOVAR, D; SKLADAL, P, 2013:PIEZOELECTRIC BIOSENSOR COUPLED TO CYCLONE AIR SAMPLER FOR DETECTION OF MICROORGANISMS. CHEMICKE LISTY 107 , p. S302 - S304.

2012

• HLAVACEK, A; SKLADAL, P, 2012:Isotachophoretic purification of nanoparticles: Tuning optical properties of quantum dots. ELECTROPHORESIS 33 (42286), p. 1427 - 1430.
• SKLADAL, P; SVABENSKA, E; ZERAVIK, J; PRIBYL, J; SISKOVA, P; TJARNHAGE, T; GUSTAFSON, I, 2012:Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols. ELECTROANALYSIS 24 (3), p. 539 - 546.

2011

• NAGY, L; ANGYAL, T; NAGY, G; AKIKO, M; PRIBYL, J; SKLADAL, P, 2011:SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress. BIODEVICES 2011 , p. 359 - 362.

2010

• CRHA, I; PRIBYL, J; SKLADAL, P; ZAKOVA, J; VENTRUBA, P; POHANKA, M, 2010:Determination of the surface pathology of human sperm by atomic force microscopy. HUMAN REPRODUCTION 25 , p. I122 - I122.
• ZERAVIK, J; LACINA, K; JILEK, M; VLCEK, J; SKLADAL, P, 2010:Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase. MICROCHIMICA ACTA 170 (3 IV), p. 251 - 256.

PROJECTS

• RIAT-CZ (ATCZ40), Interreg CZ-AT, 2016 - 2018
• CIISB4HEALTH - Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví (CZ.02.1.01/0.0/0.0/16_013/0001776), MEYS, 2017 - 2021
• CIISB - Czech Infrastructure for Integrative Structural Biology (LM2015043), MEYS, 2016 - 2019

Filter

Filtr Search Research group / CF — select — Functional Properties of Nanostructures - Josef HumlíčekSmart Nanodevices - Jaromír HubálekExperimental Biophotonics - Radim ChmelíkFabrication and Characterisation of Nanostructures - Tomáš ŠikolaDevelopment of Methods for Analysis and Measuring - Petr KlapetekMaterials Characterization and Advanced Coatings - Jozef KaiserPlasma Technologies - Lenka ZajíčkováSynthesis and Analysis of Nanostructures - Jiří PinkasMultiscale Modelling and Measurements of Physical Properties - Roman GrögerAdvanced Ceramic Materials - Martin TrunecCybernetics in Material Science - Pavel VáclavekAdvanced Polymers and Composites - Josef JančářAdvanced Metallic Materials and Metal Based CompositesGlycobiochemistry - Michaela WimmerováMolecular Microbiology - Alois ČížekZoonoses - Břetislav KoudelaOrthopaedics and Surgery - Pavel ProksAnimal Immunogenomics - Petr HořínAnimal Cytogenomics - Jiří RubešMammalian Reproduction - Martin AngerBehavioural and Social Neuroscience - Milan BrázdilApplied Neuroscience - Irena RektorováMedical Genomics - Šárka PospíšilováMolecular Oncology - Solid Cancer - Ondřej SlabýInherited Diseases - Transtricptional Regulation - Dalibor BlažekAdaptive Immunity Group - Dmitriy ChudakovBioanalytical Instrumentation - František ForetPlant Cytogenomics - Martin LysákFunctional Genomics and Proteomics of Plants - Jan HejátkoHormonal Crosstalk in Plant Development - Helene Robert BoisivonProteomics - Zbyněk ZdráhalDevelopmental and Cell Biology of Plants - Tomasz NodzynskiChromatin Molecular Complexes - Jiří FajkusDevelopmental and Production Biology – Omics Approaches - Břetislav BrzobohatýPlant Stress Signalling and Adaption - Vanesa Beatriz TognettiPlant Molecular Biology - Karel ŘíhaRNA Quality Control - Štěpánka VaňáčováNanobiotechnology - Petr SkládalRNA based regulation of Gene Expression - Peter LukavskyStructural Biology of Gene Regulation - Richard ŠteflStructural Virology - Pavel PlevkaStructure and Dynamics of Nucleic Acids - Jiří ŠponerStructure and Interaction of Biomolecules at Surfaces - Miroslav FojtaComputational Chemistry - Jaroslav KočaMulti-modal and Functional Neuroimaging - Ivan RektorCF: Core Facility GenomicsCF: Core Facility X-Ray Diffraction and Bio-SAXSCF: CEITEC NanoCF: Josef Dadok National NMR CentreCF: Core Facility Biomolecular Interaction and CrystalizationCF: Core Facility Cryo-Electron Microscopy and TomographyCF: Core Facility ProteomicsCF: Multimodal and Functional Imaging LaboratoryCF: Core Facility NanobiotechnologyStrucure of Biosystems and Molecular Materials - Radek MarekNon-Coding Genome - Lukáš TrantírekProtein Structure and Dynamics - Lukáš ŽídekProtein-DNA Interactions - Konstantinos TripsianesERA Chair - RNA and Immunity - Mary O'ConnellMicroenvironment of Immune Cells - Marek MrázCF: Cellular ImagingCF: Plant Sciences Core FacilityFunctional Genomics - Michal ŠmídaInteraction Protein-Protein and Protein-Membrane - Robert VáchaMagneto-Optical and THz Spectroscopy - Petr NeugebauerMolecular Nanostructures at Surfaces - Jan ČechalNanomagnetism and spintronics - Vojtěch Uhlíř Institution — select — CEITEC HeadquartersCEITEC Masaryk UniversityCEITEC Brno University of TechnologyCEITEC Mendel University in BrnoCEITEC University of Veterinary and Pharmaceutical Sciences BrnoCEITEC Institute of Physics of Materials, Academy of Science CZCEITEC Veterinary Research Institute Technology — select — Nanolitography infrastructureChemical & Thermal processesEtching & DepositionPackaging & TestingElectrical & Magnetic measurementsOptical measurementsProbe microscopy & NanomanipulationIn situ fabrication & CharacterizationLiquid ChromatographyElectrophoresisX-ray DiffractionX-ray ScatteringBiomolecular Interactions and characterizationBiomolecular crystallizationConfocal microscopyWidefield microscopyConfocal microscopy for live cell imagingPhytotronsSmall-size growth chambersGreenhouse

Equipment	Research group / CF	Profile card
AFM microscopy system for nanobiointeractios	CF: Core Facility Nanobiotechnology
Combined AFM / inverted optical microscope	CF: Core Facility Nanobiotechnology
Electrochemical AFM and STM module, nanolithographic module	CF: Core Facility Nanobiotechnology
Entry-level AFM microscope	CF: Core Facility Nanobiotechnology
Fluorescence imaging of surfaces with resolution 0.5-40 um/px, multi-laser excitation	CF: Core Facility Nanobiotechnology
Nanodeposition system for protein chips	CF: Core Facility Nanobiotechnology
SPR module with goniometer	CF: Core Facility Nanobiotechnology