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Advanced Nano and Microtechnologies Advanced Materials Structural Biology Gen. and Prot. of Plant Systems Molecular Medicine Brain and Mind Research Molecular Vet. Medicine

Chromatin Molecular Complexes - Jiří Fajkus

Research Group Leader
Researcher ID
Phone: +420 54949 4003
E-mail: ,
Office:
Deputy Group Leader
Researcher ID
Phone: +420 54949 8063
E-mail: ,
Office:

Research areas

  • Structure, evolution and maintenance of telomeres and their roles in chromosome stability and plant speciation; this includes the characterization of nucleoprotein composition of telomeres and telomerases, biophysical analysis of interactions between telomere components by quantitative methods, analysis of structure-function relationships of telomerase subdomains and analysis of alternative (telomerase independent) strategies of telomere maintenance
  • Epigenetic mechanisms in the regulation of gene expression, chromosome stability and telomere homeostasis
  • Structure, evolution and functions of SMC complexes; the characterization of SMC5-6 complex subunits and MAGE proteins in vitro and in vivo; their roles in DNA repair and chromosome dynamics

Main objectives

  • To investigate epigenetic processes involved in regulation of telomere maintenance
  • To clarify connections between dynamics of telomere composition and DNA damage response
  • To explore evolution of telomeres, telomerase and alternative mechanisms of telomere maintenance
  • To decipher functional links between chromatin (nucleosome) assembly and genome stability
  • To examine interactions and functions of  SMC5-6 complex subunits and MAGE proteins
     

Content of research

Chromatin is a supramolecular complex of DNA, proteins and other associated molecules (e.g. RNA species). It is the building material of chromosomes, which can be observed during cell division in their most condensed state. Chromatin was first discovered in plant cells – as were the cells themselves, or genes.

Since eukaryotic genomes are folded into chromatin, all genome functions occur in the context of this highly dynamic structure. Understanding processes such as DNA replication and repair, transcription or cell differentiation thus requires understanding structure and function of chromatin, and its specific domains like centromeres, telomeres or nucleoli.

While the nucleotide sequence of the DNA component of chromatin constitutes the genetic material of the cell, the other chromatin components (and also modifications of bases in DNA itself) participate in so-called epigenetic functions. These include spatiotemporal regulation of gene activity and DNA replication, correct and precise segregation of genetic material to daughter cells, maintenance of chromosome stability, and protection of genetic material from damage. Importantly, the chromatin structure compacts several metres of genomic DNA to fit the size of a cell nucleus (several microns in diameter) while keeping it functional despite the high degree of compaction (105-106 fold).

Our research group integrates studies in the field of telomere biology, chromatin structure and epigenetics. Using unique features of plant systems (namely their high developmental plasticity), and their comparison to yeast or animal models, we aim to characterise pathways involved in control of chromosome stability and distinguish between specific and general mechanisms involved. Outcomes of our studies (e.g., understanding mechanisms contributing to genome stability, aging or adaptation to changing environmental conditions) can be applied in agriculture, biotechnologies or medicine.

list / cards

Name and position

E-mail

Phone

Prof. Jiří Fajkus
Research Group Leader
+420 54949 4003
Jan Paleček
Senior Researcher
+420 54949 6128
Miloslava Fojtová
Researcher
+420 54949 8063
Ctirad Hofr, Ph.D.
Researcher
+420 54949 5952
Dagmar Zachová
Researcher
+420 54949 5601
Martina Dvořáčková, Ph.D.
Postdoctoral Fellow
+420 54949 5403
Kateřina Havlová
PhD student
+420 54949 5354
Ivona Nečasová
PhD student
+420 54949 8155
Lucie Vondrová
PhD student
+420 54949 5863
Lucie Němcová
Assistant
+420 54949 4482
Monika Chropovská
laborantka
+420 54949 2682
Kateřina Jůzová
PhD student
+420 54949 5863
Jana Kapustová
Technician
+420 54949 1494
Martin Lyčka
odborný pracovník - PhD student
Konstantin Kutashev
odborný pracovník - PhD student
+420 54949 8139
Tomáš Crhák
PhD student
+420 54949 2655
Martina Nešpor Dadejová, Ph.D.
odborná pracovnice ve výzkumu
+420 54949 2655
Markéta Pernisová, Ph.D.
Researcher
+420 54949 6470
Edita Balkóová
+420 54949 5863
Petr Fajkus
PhD student
+420 54949 2655
Lenka Jurčišinová
PhD student
+420 54949 5863
Jan Špaček
PhD student
Martin Stojaspal
Student
Pavel Veverka
PhD student
Marek Adamus
PhD student
Tomáš Janovič

SELECTED PUBLICATIONS

2017

  • BRABENCOVA, S; IHNATOVA, I; POTESIL, D; FOJTOVA, M; FAJKUS, J; ZDRAHAL, Z; LOCHMANOVA, G, 2017:Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors. FRONTIERS IN PLANT SCIENCE 8
  • DOBISOVA, T; HRDINOVA, V; CUESTA, C; MICHLICKOVA, S; URBANKOVA, I; HEJATKOVA, R; ZADNIKOVA, P; PERNISOVA, M; BENKOVA, E; HEJATKO, J, 2017:Light Controls Cytokinin Signaling via Transcriptional Regulation of Constitutively Active Sensor Histidine Kinase CKI1. PLANT PHYSIOLOGY 174 (1), p. 387 - 404.
  • JURECKOVA, JF; SYKOROVA, E; HAFIDH, S; HONYS, D; FAJKUS, J; FOJTOVA, M, 2017:Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum. PLANTA 245 (3), p. 549 - 561.
  • NECASOVA, I; JANOUSKOVA, E; KLUMPLER, T; HOFR, C, 2017:Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it (gkx812) (vol 45, pg 12170, 2017). NUCLEIC ACIDS RESEARCH 45 (21), p. 12599 - 12599.
  • NECASOVA, I; JANOUSKOVA, E; KLUMPLER, T; HOFR, C, 2017:Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. NUCLEIC ACIDS RESEARCH 45 (21), p. 12170 - 12180.
  • VALUCHOVA, S; FULNECEK, J; PROKOP, Z; STOLT-BERGNER, P; JANOUSKOVA, E; HOFR, C; RIHA, K, 2017:Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. PLANT CELL 29 (6), p. 1533 - 1545.

2016

  • FAJKUS, P; PESKA, V; SITOVA, Z; FULNECKOVA, J; DVORACKOVA, M; GOGELA, R; SYKOROVA, E; HAPALA, J; FAJKUS, J, 2016:Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase. PLANT JOURNAL 85 (3), p. 337 - 347.
  • HAVLOVA, K; DVORACKOVA, M; PEIRO, R; ABIA, D; MOZGOVA, I; VANSACOVA, L; GUTIERREZ, C; FAJKUS, J, 2016:Variation of 45S rDNA intergenic spacers in Arabidopsis thaliana. PLANT MOLECULAR BIOLOGY 92 (2016-05-04T00:00:00.000), p. 457 - 471.
  • NODZYNSKI, T; VANNESTE, S; ZWIEWKA, M; PERNISOVA, M; HEJATKO, J; FRIML, J, 2016:Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. MOLECULAR PLANT 9 (11), p. 1504 - 1519.
  • PERNISOVA, M; PRAT, T; GRONES, P; HARUSTIAKOVA, D; MATONOHOVA, M; SPICHAL, L; NODZYNSKI, T; FRIML, J; HEJATKO, J, 2016:Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. NEW PHYTOLOGIST 212 (2), p. 497 - 509.
  • SEPSIOVA, R; NECASOVA, I; WILLCOX, S; PROCHAZKOVA, K; GORILAK, P; NOSEK, J; HOFR, C; GRIFFITH, JD; TOMASKA, L, 2016:Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. PLOS ONE 11 (4)
  • SCHRUMPFOVA, PP; VYCHODILOVA, I; HAPALA, J; SCHOROVA, S; DVORACEK, V; FAJKUS, J, 2016:Telomere binding protein TRB1 is associated with promoters of translation machinery genes in vivo. PLANT MOLECULAR BIOLOGY 90 (2016-02-01T00:00:00.000), p. 189 - 206.
  • VAN DER CRABBEN, SN; HENNUS, MP; MCGREGOR, GA; RITTER, DI; NAGAMANI, SCS; WELLS, OS; HARAKALOVA, M; CHINN, IK; ALT, A; VONDROVA, L; HOCHSTENBACH, R; VAN MONTFRANS, JM; TERHEGGEN-LAGRO, SW; VAN LIESHOUT, S; VAN ROOSMALEN, MJ; RENKENS, I; DURAN, K; NIJMAN, IJ; KLOOSTERMAN, WP; HENNEKAM, E; ORANGE, JS; VAN HASSELT, PM; WHEELER, DA; PALECEK, JJ; LEHMANN, AR; OLIVER, AW; PEARL, LH; PLON, SE; MURRAY, JM; VAN HAAFTEN, G, 2016:Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease. JOURNAL OF CLINICAL INVESTIGATION 126 (8), p. 2881 - 2892.
  • ZABRADY, K; ADAMUS, M; VONDROVA, L; LIAO, C; SKOUPILOVA, H; NOVAKOVA, M; JURCISINOVA, L; ALT, A; OLIVER, AW; LEHMANN, AR; PALECEK, JJ, 2016:Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. NUCLEIC ACIDS RESEARCH 44 (3), p. 1064 - 1079.

2015

  • DOKLADAL, L; HONYS, D; RANA, R; LEE, LY; GELVIN, SB; SYKOROVA, E, 2015:cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase. FRONTIERS IN PLANT SCIENCE 6
  • DVORACKOVA, M; FOJTOVA, M; FAJKUS, J, 2015:Chromatin dynamics of plant telomeres and ribosomal genes. PLANT JOURNAL 83 (1), p. 18 - 37.
  • FOJTOVA, M; SYKOROVA, E; NAJDEKROVA, L; POLANSKA, P; ZACHOVA, D; VAGNEROVA, R; ANGELIS, KJ; FAJKUS, J, 2015:Telomere dynamics in the lower plant Physcomitrella patens. PLANT MOLECULAR BIOLOGY 87 (6), p. 591 - 601.
  • HEGER, Z; MICHALEK, P; GURAN, R; HAVELKOVA, B; KOMINKOVA, M; CERNEI, N; RICHTERA, L; BEKLOVA, M; ADAM, V; KIZEK, R, 2015:Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida. PLOS ONE 10 (12)
  • JANOUSKOVA, E; NECASOVA, I; PAVLOUSKOVA, J; ZIMMERMANN, M; HLUCHY, M; MARINI, V; NOVAKOVA, M; HOFR, C, 2015:Human Rap1 modulates TRF2 attraction to telomeric DNA. NUCLEIC ACIDS RESEARCH 43 (5), p. 2691 - 2700.
  • KOZAKOVA, L; VONDROVA, L; STEJSKAL, K; CHARALABOUS, P; KOLESAR, P; LEHMANN, AR; ULDRIJAN, S; SANDERSON, CM; ZDRAHAL, Z; PALECEK, JJ, 2015:The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. CELL CYCLE 14 (6), p. 920 - 930.
  • MUCHOVA, V; AMIARD, S; MOZGOVA, I; DVORACKOVA, M; GALLEGO, ME; WHITE, C; FAJKUS, J, 2015:Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants. PLANT JOURNAL 81 (2), p. 198 - 209.
  • PALECEK, E; TKAC, J; BARTOSIK, M; BERTOK, T; OSTATNA, V; PALECEK, J, 2015:Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics. CHEMICAL REVIEWS , p. 20452045 - 2108.
  • PESKA, V; FAJKUS, P; FOJTOVA, M; DVORACKOVA, M; HAPALA, J; DVORACEK, V; POLANSKA, P; LEITCH, AR; SYKOROVA, E; FAJKUS, J, 2015:Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome. PLANT JOURNAL 82 (4), p. 644 - 654.
  • TRAN, TD; CAO, HX; JOVTCHEV, G; NEUMANN, P; NOVAK, P; FOJTOVA, M; VU, GTH; MACAS, J; FAJKUS, J; SCHUBERT, I; FUCHS, J, 2015:Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea. PLANT JOURNAL 84 (6), p. 1087 - 1099.
  • ZDARSKA, M; DOBISOVA, T; GELOVA, Z; PERNISOVA, M; DABRAVOLSKI, S; HEJATKO, J, 2015:Illuminating light, cytokinin, and ethylene signalling crosstalk in plant development. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 4913 - 4931.

2014

  • MAJEROVA, E; MANDAKOVA, T; VU, GTH; FAJKUS, J; LYSAK, MA; FOJTOVA, M, 2014:Chromatin features of plant telomeric sequences at terminal vs. internal positions. FRONTIERS IN PLANT SCIENCE 5 , p. 593 - .
  • OGROCKA, A; POLANSKA, P; MAJEROVA, E; JANEBA, Z; FAJKUS, J; FOJTOVA, M, 2014:Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. NUCLEIC ACIDS RESEARCH 42 (5), p. 2919 - 2931.
  • SCHRUMPFOVA, PP; VYCHODILOVA, I; DVORACKOVA, M; MAJERSKA, J; DOKLADAL, L; SCHOROVA, S; FAJKUS, J, 2014:Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. PLANT JOURNAL 77 (5), p. 770 - 781.

2013

  • CINCAROVA, L; ZDRAHAL, Z; FAJKUS, J, 2013:New perspectives of valproic acid in clinical practice. EXPERT OPINION ON INVESTIGATIONAL DRUGS 22 (12), p. 1535 - 1547.

2012

  • CINCAROVA, L; LOCHMANOVA, G; NOVAKOVA, K; SULTESOVA, P; KONECNA, H; FAJKUSOVA, L; FAJKUS, J; ZDRAHAL, Z, 2012:A combined approach for the study of histone deacetylase inhibitors. MOLECULAR BIOSYSTEMS 8 (11), p. 2937 - 2945.
  • GUERINEAU, M; KRIZ, Z; KOZAKOVA, L; BEDNAROVA, K; JANOS, P; PALECEK, J, 2012:Analysis of the Nse3/MAGE-Binding Domain of the Nse4/EID Family Proteins. PLOS ONE 7 (4)
  • KUBICEK, K; CERNA, H; HOLUB, P; PASULKA, J; HROSSOVA, D; LOEHR, F; HOFR, C; VANACOVA, S; STEFL, R, 2012:Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1. GENES & DEVELOPMENT 26 (17), p. 1891 - 1896.
  • LEE, LY; WU, FH; HSU, CT; SHEN, SC; YEH, HY; LIAO, DC; FANG, MJ; LIU, NT; YEN, YC; DOKLADAL, L; SYKOROVA, E; GELVIN, SB; LIN, CS, 2012:Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. PLANT CELL 24 (5), p. 1746 - 1759.
  • OGROCKA, A; SYKOROVA, E; FAJKUS, J; FOJTOVA, M, 2012:Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. JOURNAL OF EXPERIMENTAL BOTANY 63 (11), p. 4233 - 4241.
  • POLANSKA, E; DOBSAKOVA, Z; DVORACKOVA, M; FAJKUS, J; STROS, M, 2012:HMGB1 gene knockout in mouse embryonic fibroblasts results in reduced telomerase activity and telomere dysfunction. CHROMOSOMA 121 (4), p. 419 - 431.
  • VISACKA, K; HOFR, C; WILLCOX, S; NECASOVA, I; PAVLOUSKOVA, J; SEPSIOVA, R; WIMMEROVA, M; SIMONICOVA, L; NOSEK, J; FAJKUS, J; GRIFFITH, JD; TOMASKA, L, 2012:Synergism of the Two Myb Domains of Tay1 Protein Results in High Affinity Binding to Telomeres. JOURNAL OF BIOLOGICAL CHEMISTRY 287 (38), p. 32206 - 32215.

2011

  • FOJTOVA, M; PESKA, V; DOBSAKOVA, Z; MOZGOVA, I; FAJKUS, J; SYKOROVA, E, 2011:Molecular analysis of T-DNA insertion mutants identified putative regulatory elements in the AtTERT gene. JOURNAL OF EXPERIMENTAL BOTANY 62 (15), p. 5531 - 5545.
  • HOBOR, F; PERGOLI, R; KUBICEK, K; HROSSOVA, D; BACIKOVA, V; ZIMMERMANN, M; PASULKA, J; HOFR, C; VANACOVA, S; STEFL, R, 2011:Recognition of Transcription Termination Signal by the Nuclear Polyadenylated RNA-binding (NAB) 3 Protein. JOURNAL OF BIOLOGICAL CHEMISTRY 286 (5), p. 3645 - 3657.
  • HUDSON, JJR; BEDNAROVA, K; KOZAKOVA, L; LIAO, CY; GUERINEAU, M; COLNAGHI, R; VIDOT, S; MAREK, J; BATHULA, SR; LEHMANN, AR; PALECEK, J, 2011:Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families. PLOS ONE 6 (2)
  • MAJEROVA, E; FOJTOVA, M; MOZGOVA, I; BITTOVA, M; FAJKUS, J, 2011:Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells. PLANT MOLECULAR BIOLOGY 77 (42128), p. 371 - 380.

2010

  • RICHARD STEFL, FLORIAN C. OBERSTRASS, JENNIFER L. HOOD, MURIEL JOURDAN, MICHAL ZIMMERMANN, LENKA SKRISOVSKA, CHRISTOPHE MARIS, LI PENG, CTIRAD HOFR, RONALD B. EMESON, FRÉDÉRIC H.-T. ALLAINE, 2010:The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove. CELL 143 (2), p. 225 - 237.

2009

  • HOFR, C., SULTESOVA, P., ZIMMERMANN, M., MOZGOVA, I., SCHRUMPFOVA, P. P., WIMMEROVA, M., FAJKUS, J.,, 2009:Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specificity and kinetics. BIOCHEMICAL JOURNAL 419 , p. 221 - 228.

GRANTY

  • Dynamics of shelterin complex assembly (P205/12/0550), Czech Science Foundation - Standard Grants, 2012 - 2015
  • Telomeres and telomerase: transition from molecular to structural biology approach (IAA500040801), Academy of Sciences of the Czech Republic - Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Rep., 2008 - 2012
  • Epigenetic mechanisms of plant telomeres regulation (GAP501/11/0569), Czech Science Foundation - Standard Grants, 2011 - 2015
  • Loss of specific DNA repeats in response to dysfunction of CAF1 in plants (GAP501/11/0289), Czech Science Foundation - Standard Grants, 2011 - 2015

CURRENT RESEARCH INFRASTRUCTURE

The current research infrastructure includes high-end microscopy, quantitative fluorescence spectroscopy, centrifugation and ultracentrifugation, preparative and analytical chromatography, electrophoresis, in situ and Southern hybridization, phosphofluoroimaging, real-time PCR and a documentation system for chemiluminiscence and fluorescence.

1. SMC complexes dynamics

Supervisor: doc. Mgr. Jan Paleček, Dr. rer. nat.

Annotation

The SMC (Structure Maintenance of Chromosome) complexes are the key components of higher-order chromatin fibers and play important roles in genome stability. Three SMC complexes are present in most eukaryotic cells: cohesin (SMC1/3), condensin (SMC2/4) and SMC5/6 complex. Cohesin can make internal loops or embrace two sister chromatids (feature essential for proper chromosome segregation); condensin interconnects loops to condense chromatin during mitosis. The SMC5/6 complex is involved in the homologous recombination-based DNA repair, in replication fork stability and processing, and in cohesin regulation. In our lab, we study assembly and functions of SMC5/6 complexes (http://www.ncbr.muni.cz/SPEC/). New student will use combination of genetic (fission yeast model), biochemical (mostly yeast two-hybrid system and other binding assays) and bioinformatics methods to get deep insights into SMC5/6 features.

Keywords: Structure Maintenance of Chromosome, SMC complexes, SMC5/6; genome stability, chromatin fibers

2. Structure, function and evolution of plant telomere components

Supervisor: prof. RNDr. Jiří Fajkus, CSc.

Annotation

Telomeres are terminal, but nevertheless integral parts of chromosomes, and as such they are also formed and function as supramolecular nucleoprotein (chromatin) structures. Their DNA component is usually formed by repetitive DNA whose incomplete end-replication by the conventional replication machinery can be compensated by elongation via a specific ribonucleoprotein complex with RNA-dependent DNA polymerase activity – the telomerase. Besides preventing the replicative shortening, telomeres also protect chromosome ends from being mistaken for unrepaired chromosome breaks. For the latter function, protein components of telomeres are responsible. Our group has described evolutionary changes in plant telomere DNA repeats and some protein components of plant telomeres in typical telomeres in the model system of Arabidopsis thaliana. It will be important to follow-up these studies with description of further protein components of telomeres together with testing their functional importance for telomere maintenance and chromosome stability. In addition, the impact of the evolutionary change in telomere DNA sequence on its protein interactors will be examined. Background in molecular biology, biochemistry and bioinformatics will be an asset for selection of a suitable candidate. Literature: Fajkus, P., Peska, V., Sitova, Z., Fulneckova, J., Dvorackova, M., Gogela, R., Sykorova, E., Hapala, J. and Fajkus, J. (2016) Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)n is synthesized by telomerase. Plant J, 85, 337-347. Prochazkova Schrumpfova, P., Schorova, S. and Fajkus, J. (2016) Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell. Front Plant Sci, 7, 851. Schrumpfova, P.P., Vychodilova, I., Dvorackova, M., Majerska, J., Dokladal, L., Schorova, S. and Fajkus, J. (2014) Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. Plant J, 77, 770-781.

Keywords: plant telomere DNA repeats, telomere maintenance, telomerase

3. The role of cytokinins in determination of organ identity.

Supervisor: Mgr. Markéta Pernisová, Ph.D.
Consultants: prof. RNDr. Jiří Fajkus, CSc., Helene Robert Boisivon, Ph.D.

Annotation

Plants, in comparison to animals, are unique in a developmental plasticity that allows their adaptation to changing environmental conditions. Plants undergo postembryonic de novo organogenesis and can continually regenerate organs. This ability is a critically important developmental adaptation in plants. The phytohormones auxin and cytokinin are thought to be major regulators of plant organogenesis. Auxin is necessary to induce de novo organ development, while cytokinins modulate the organogenic response resulting in root or shoot formation. Although the function of several members of cytokinin signalling pathway has already been described, their role in the determination of organ identity is still elusive. The goal of the project will be i) to analyse cytokinin effect in de novo organogenesis and ii) to identify and analyse regulatory factors important for that process. We expect: creativity, self-reliance, willingness to learn new approaches. We offer: experimental work on the project, excellent lab equipment, high-end confocal microscopes, help with both intellectual and practical issues, friendly lab staff, attractive environment of university campus, competitive salary corresponding to the work efficiency.

Keywords: cytokinin signalling, plant organogenesis, organ identity

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