Web: | vacha.ceitec.cz/ |
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Our aim is to elucidate the key protein-membrane and protein-protein interactions that regulate cellular signaling, transport, and are the important part of our immune system. Change in these interactions can result in lethal diseases ranging from cancer to Alzheimer’s disease. In particular, we develop and apply multi-scale computer simulations (from highly coarse-grained to all-atom level) and theory to determine the relation between protein sequence and their assembly or preferred membrane composition and morphology, e.g. the aggregation of amyloid peptides enhanced or retarded by specific lipids. Such molecular information can be used to understand protein localization in cells, evaluate the risk of the diseases, develop new therapeutic peptides (antimicrobial peptides, sensors, target specific drug carriers), and suggest new treatment strategies (including lipid diet and altering lipid metabolism).
list / cards
Name and position |
Phone |
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Miroslav Jurásek PhD student |
+420 54949 2524 | |
Ivo Kabelka PhD student |
+420 54949 2524 | |
Lukáš Sukeník PhD student |
+420 54949 2524 | |
Robert Vácha, PhD. Research Group Leader |
+420 54949 6846 | |
Francesco Luca Falginella PhD student |
+420 54949 8140 | |
Tereza Králová laborantka |
+420 54949 3651 | |
Rahul Deb odborný pracovník - PhD student |
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Arnošt Mládek, Ph.D. Postdoctoral Fellow |
+420 54949 5398 |
Supervisor: doc. RNDr. Robert Vácha, PhD.
Peptide and protein conformations are influenced by composition of phospholipid membrane, which they are interacting with. This is particularly important for antimicrobial and neurodegenerative peptides (such as Abeta peptide related to Alzheimer’s disease), which can disrupt cellular membrane by formation of toxic membrane pores. The lipid composition of the membrane can enhance or prevent the peptide aggregation and/or pore formation and thus determine the fate of the cell. Despite the importance, we do not understand the relation except few recent examples (e.g. DOI: 10.1002/anie.201603178). The aim of this project is to unravel the relationship between the membrane composition and peptide conformations. This information could lead to a new diet preventing the Alzheimer’s disease or to a rational design of new antimicrobial peptides. Student will master tools of computer simulations, in particular molecular dynamics technique, and methods to calculate free energies. Moreover, he/she will learn advantages and disadvantages of various protein and membrane parameterizations including all-atom and coarse-grained models.
Keywords: protein conformations, phospholipid membrane, neurodegenerative peptides, Alzheimer’s disease, Abeta peptide, peptide conformations, membrane parameterizations
Supervisor: doc. RNDr. Robert Vácha, PhD.
The control of biological membrane shape and composition is vital to eukaryotic life. The shape is so specific that it allows us to recognize various organelles in the cell. Despite a continuous exchange of material, organelles maintain a precise combination and organization of membrane lipids, which is crucial for their function and the recruitment of many peripheral proteins. Membrane shape thus enables the cell to organize proteins and their functions in space and time, without which serious diseases can occur. Moreover, membrane curvature and lipid content can be specific to cancer cells, bacteria, and enveloped virus coatings, which could be utilized for selective targeting. The aim of this project is to unravel the relationship between the protein sequence and the preferred membrane. This will lay the foundations for the design of new protein motifs sensitive to membranes with a specific curvature and composition on enveloped viruses, organelles and cells. Student will master tools of computer simulations, in particular molecular dynamics technique, and methods to calculate free energies. Moreover, he/she will learn advantages and disadvantages of various protein and membrane parameterizations including all-atom and coarse-grained models.
Keywords: membrane curvature, protein sequencing, protein motifs design, membrane parameterizations
29. ledna 2018 9:46
LECTURE: Dr. Ondrej Hovorka: Models of magnetic nanoparticles for biomedical applications
25. ledna 2018 18:21
WHEN: 30. 01. 2018 WHERE: CEITEC BUT, Purkynova 123, large meeting room SPEAKER: Dr Andriy Marko TALK: Advances in PELDOR…